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M94A0743.TXT
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1994-10-21
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Document 0743
DOCN M94A0743
TI Lymphocyte subset analysis by Boolean algebra: a phenotypic approach
using a cocktail of 5 antibodies and 3 colour immunofluorescence.
DT 9412
AU Hunter S; Peters L; Wotherspoon J; Crowe S; Flow Cytometry Unit,
Macfarlane Burnet Centre for Medical; Research, Fairfield Hospital,
Victoria, Australia.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 52).
Unique Identifier : AIDSLINE ASHM5/94348912
AB A rapid method has been developed whereby total CD3+ T-cells, CD4+
T-cells (CD3+CD4+), CD8+ T-cells (CD3+CD8+), putative gamma
delta-receptor-T-cells (CD3+CD4-CD8-) and T-cells that are CD3+CD4+CD8+
as well as B-lymphocytes and NK-cells can be enumerated after staining
in a single tube. Whole blood specimens are labelled with a mixture of
antibodies: FITC-CD4 and CD19, PE-CD8 and CD16, and either peridinin
chlorophyll protein (PerCP) or allophycocyanin (APC) labelled CD3 for
use on a Becton Dickinson FACScan or FACStar Plus flow cytometer
respectively. Data were analysed with LYSYS-II software package and all
of the lymphocyte subset values were determined by Boolean algebra. Our
study has shown that this new procedure is statistically equivalent to a
standard analysis procedure (SimulSET lymphocyte subset analysis), is
less time consuming and more cost effective.
DE B-Lymphocyte Subsets/*IMMUNOLOGY Flow Cytometry *Fluorescent Antibody
Technique Human Immunophenotyping/*METHODS Leukocyte Count/*METHODS
Software T-Lymphocyte Subsets/*IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).